I-Cortistatin-29 (igundane)/1815618-17-7/GT Peptide/Peptide Supplier

Incazelo

I-Corticostatin 29 iyi-neuropeptide efana nesakhiwo efana ne-somatostatin 28. 1 Ikhiqizwa ngokuqhekeka kwe-procortatin ku-procortatin, ecwenga kuma-amino acid anambambili ukwenza i-cortatin 29 ne-cortatin 14 neminye imikhiqizo ye-cleavage ingxenye.I-Corticostatin mRNA ivezwa ebuchosheni bomuntu kanye nakuma-interneurons ku-hippocampus kanye ne-cerebral cortex yamagundane.1, 2 Cortistatin-29 eboshelwe kumamukeli we-somatostatin (SST) onamanani e-IC 50 angu-2.8, 7.1, 0.2, 3 kanye no-13.7 nM we-SST 1-5, ngokulandelanayo.2 ithole amazinga afanayo e-corstatin 29 kuya ku-corstatin 14 kumaseli e-AtT20 egundane, kodwa emazingeni aphansi okukhishwa.I-Cortistatin-29 ihambisana nezinsalela ezingama-85 kuye kwezingu-112 zokulandelana kwe-peptide yamagundane.

Imininingwane

Ukubukeka: Impushana emhlophe ukuya kokumhlophe

Ubumsulwa(HPLC):≥98.0%

Ukungcola Okukodwa:≤2.0%

Okuqukethwe kwe-Acetate(HPLC): 5.0%～12.0%

Okuqukethwe Amanzi (Karl Fischer):≤10.0%

Okuqukethwe kwe-Peptide:≥80.0%

Ukupakisha Nokuthumela: Izinga lokushisa eliphansi, ukupakisha kwe-vacuum, kunembile ku-mg njengoba kudingeka.

Unga-oda Kanjani?

1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.

2. Oda ku-inthanethi.Sicela ugcwalise ifomu le-oda ku-inthanethi.

3. Nikeza igama le-peptide, Inombolo ye-CAS noma ukulandelana, ukuhlanzeka nokuguqulwa uma kudingeka, ubuningi, njll. sizohlinzeka ngekhotheshini kungakapheli amahora ama-2.

4. Ukugcinwa kwe-oda ngenkontileka yokuthengisa esayiniwe ngokufanelekile kanye ne-NDA(isivumelwano esingadalulwanga) noma isivumelwano esiyimfihlo.

5. Sizoqhubeka nokubuyekeza inqubekelaphambili ye-oda ngesikhathi.

6. Ukulethwa kwe-Peptide nge-DHL, i-Fedex noma abanye, kanye ne-HPLC, MS, COA izohlinzekwa kanye nempahla.

7. Inqubomgomo yembuyiselo izolandelwa uma kukhona ukushayisana kwekhwalithi noma isevisi yethu.

8. Isevisi yangemuva kokuthengisa: Uma amakhasimende ethu enemibuzo mayelana ne-peptide yethu ngesikhathi sokuhlolwa, sicela ukhululeke ukusithinta futhi sizoyiphendula ngokushesha.

Yonke imikhiqizo yenkampani isetshenziselwa inhloso yocwaningo lwesayensi kuphela, yona'kwenqatshelwe ukusetshenziswa ngokuqondile yinoma yimuphi umuntu emzimbeni womuntu.

FAQ:

Ingabe ama-peptide aqukethe ama-Cys ancishisiwe ngaphambi kokuthunyelwa?

Uma i-peptide ingatholakali ukuthi ifakwe i-oxidized, ngokuvamile asinciphisi ama-Cys.Wonke ama-polypeptide atholakala emikhiqizweni engcolile ehlanjululwe futhi i-lyophilized ngaphansi kwezimo ze-pH2, okuthi okungenani ngokwezinga elithile ivimbele ukukhishwa kwe-Cys.Ama-Peptide aqukethe ama-Cys ahlanzwa ku-pH2 ngaphandle uma kunesizathu esithile sokuhlanza ku-pH6.8.Uma ukuhlanzwa kwenziwa ku-pH6.8, umkhiqizo ohlanziwe kufanele uphathwe nge-asidi ngokushesha ukuze uvimbele ukugcotshwa kwe-oxidation.Esinyathelweni sokugcina sokulawula ikhwalithi, kuma-peptide aqukethe ama-Cys, uma ubukhona besisindo samangqamuzana (2P+H) butholakala kumephu ye-MS, kukhombisa ukuthi i-dimer yenziwe.Uma ingekho inkinga nge-MS kanye ne-HPLC, sizoziphilisa ngokuqondile futhi sithumele izimpahla ngaphandle kokuqhubeka nokucutshungulwa.Kufanele kuqashelwe ukuthi ama-peptide aqukethe ama-Cys athola i-oxidation ehamba kancane ngokuhamba kwesikhathi, futhi izinga le-oxidation lincike ekulandeleni kwe-peptide nezimo zokugcinwa.

Unquma kanjani ukuthi i-peptide i-looped?

Sisebenzisa ukusabela kuka-Ellman ukuhlola ukuthi ukwakheka kweringi kuqediwe yini.Uma ukuhlolwa kuka-Ellman ku-positive (okuphuzi), ukusabela kwendandatho akuphelele.Uma imiphumela yokuhlolwa inegethivu (hhayi ephuzi), ukusabela kwendandatho sekuqediwe.Asinikezi ngombiko wokuhlaziya wokuhlonza ibhayisikili kumakhasimende ethu.Ngokuvamile, kuzoba nencazelo yemiphumela yokuhlolwa kuka-Ellman embikweni we-QC.

Ngidinga i-cyclic peptide, equkethe i-tryptophan, ingabe izokwenziwa i-oxidized?

I-oxidation ye-tryptophan iyinto evamile ku-peptide oxidation, futhi ama-peptide avame ukuqhutshwa ngamabhayisikili ngaphambi kokuhlanzwa.Uma i-oxidation ye-tryptophan yenzeka, isikhathi sokugcinwa kwe-peptide kukholomu ye-HPLC sizoshintsha, futhi i-oxidation ingasuswa ngokuhlanzwa.Ngaphezu kwalokho, ama-peptide ane-oxidized nawo angatholwa yi-MS.

Ingabe kuyadingeka ukubeka igebe phakathi kwe-peptide nodayi?

Uma uzonamathisela i-molecule enkulu (njengodayi) ku-peptide, kungcono kakhulu ukubeka isikhala phakathi kwe-peptide ne-ligand ukuze unciphise ukuphazamiseka kwesamukeli ngokugoqa i-peptide ngokwayo noma ngokugoqa inhlanganisela yayo.Abanye abazifuni izikhawu.Isibonelo, ekugoqeni amaprotheni, kungenzeka ukunquma ukuthi ukuqhelelana kangakanani isakhiwo esigoqayo se-amino acid ngokunamathisela udayi we-fluorescent endaweni ethile.

Uma ufuna ukwenza ukuguqulwa kwe-biotin kutheminali engu-N, ingabe udinga ukubeka igebe phakathi kwe-biotin nokulandelana kwe-peptide?

Inqubo evamile yokulebula ye-biotin esetshenziswa inkampani yethu ukunamathisela i-Ahx ochungechungeni lwe-peptide, ilandelwe yi-biotin.I-Ahx iyinhlanganisela yekhabhoni engu-6 esebenza njengesithiyo phakathi kwe-peptide ne-biotin.

Ungakwazi yini ukunikeza iseluleko mayelana nokwakhiwa kwama-peptide e-phosphorylated?

Njengoba ubude bukhula, ukusebenza kahle kokubophezela kuyehla kancane kancane kusuka ku-amino acid e-phosphorylated kuye phambili.Isiqondiso se-synthesis sisuka ku-C terminal kuya ku-N terminal.Kutuswa ukuthi izinsalela ngemva kwe-amino acid ene-phosphorylated akumele zidlule ku-10, okungukuthi, inani lezinsalela ze-amino acid ngaphambi kwe-amino acid eyi-phosphorylated esuka ku-N terminal kuya ku-C terminal akufanele idlule ku-10.

Kungani i-n-terminal acetylation kanye ne-C-terminal amidation?

Lezi zinguquko zivimbela i-peptide ekonakalisweni futhi ivumela i-peptide ukuthi ilingise isimo sayo sangempela samaqembu e-alpha amino ne-carboxyl kuphrotheni engumzali.