I-Peptide YangokwezifisoI-synthesisIsevisi
I-Hangzhou Go Top Peptide Biotech , eyasungulwa ngo-2014, i-a I-National High-tech Enterpriseonguchwepheshe ocwaningweni, ekuthuthukisweni nasekukhiqizweni kwama-peptide kanye nokuphuma okuhlobene.
Eyenkampaniisikhungo socwaningo lwe-peptideokwamanje itholakala esifundeni sase-Qiantangof I-Hangzhou, ehlanganisa indawo engaphezu kuka-3,000 square metres.Iinkampani's ifektri itholakala ku-No.24, Haitian Road, Shangyu District, idolobha laseShaoxing, esifundazweni saseZhejiang.Kukhona futhi izitshalo ezimbili zokubambisana, enye ngeyeukukhiqizwa kwe-peptide ye-GMP etholakala kuIsifunda sase-Anji esifundazweni saseZhejiang, eyodwa eye-molecule encane ye-API etholakala kuyoIdolobha laseYuncheng esifundazweni saseShanxi.Sibaibhizinisi lobuchwepheshe obuphezulu obuphelele, ukuhlanganisa ucwaningo, ukulinganisa ukulinganisa, ukuthengisa nokuthengisa emhlabeni jikelele.
Inkampani inakhophezu100abasebenzi,futhi uyeithuthukiswe ngaphezu40 imithi evuthiweama-peptides,Ngaphezu kwe10 amalungelo obunikazi bokusungulwazigunyaziwe.Tumthamo wonyaka wokukhiqizais kuya ku100 kilograms, inani lama-peptide angokwezifiso analo ifinyelele ngaphezu kwezi-20,000.Singahlinzeka ngamasevisi e-peptide okuma okukodwa ukusuka kucwaningo(mg) kuya ekuhwebeni (KGs).
Uhlu Lokuguqulwa Kwe-Peptide
N-ukulungiswa kwetheminali
| {N-Me-Ala} | {N-Me-Asp} | {N-Me-Glu} | {N-Me-Ile} |
| {N-Me-Leu} | {N-Me-Met} | {N-Me-Nle} | {N-Me-Nva} |
| {N-Me-Phe} | {N-Me-Ser} | {N-Me-Thr} | {N-Me-Tyr} |
| {N-Me-Val} | {Sar} | I-N-Me-Arg | N-Me-Asn |
| N-Me-beta-Ala | I-N-Me-Gln | N-Me-Ls | I-N-Me-Trp |
C-ukulungiswa kwetheminali
| {Ibhuloho le-Mono Disulfide} | {Ibhuloho le-Double Disulfide} | {Ibhuloho le-Triple Disulfide} |
| {Ibhuloho le-Disulfide elingahleliwe} | {Same Seq.Ibhuloho le-Inter-Disulfide } | {Ibhuloho elihlukile le-Inter-Disulfide} |
| {I-Amide cyclic (ekupheleni)} | {Amide cyclic (Side chain)} | {Orn side chain Amide cyclic} |
| {Thioester cyclic} | {lactone cyclic} | {Sec cyclic} |
Ama-amino acid angajwayelekile
| {2-Aze} | {3-Aze} | {5,5-Dime-Pro} |
| {5-ASA} | {Abu} | {Ac-Ls} |
| {Ayib} | {Beta-Asp} | {Bpa} |
| {Cha} | {Chg} | {Cit} |
| {Cpg} | {Cys(Acm)} | {Cys(Bzl)} |
I-Isotope ebizwa ngokuthi ama-peptides
| I-Lys(13C6,15N2) | I-Ile(13C6,15N) |
| I-Leu(13C6,15N) | I-Val(13C5, 15N) |
| I-Arg(13C6, 15N4) |
Ukuguqulwa kwe-Fluorescent peptide/amapheya weFRET
| I-Biotin- | Dansyl- | I-peptide ye-fluorecent ecishiwe |
| I-Biotin-Ahx- | I-Dansyl-Ahx- | Dabcyl |
| 5-FAM- | Cy3- | I-Dabcyl / Glu(Edans)-NH2 |
| 5-FAM-Ahx- | Cy5- | Dansyl- |
| FITC- | I-MCA/DNP | I-Dansyl-Ahx- |
| I-FITC-Ahx- | I-AMC | Edans / Dabcyl |
I-Peptide Conjugates
| I-BSA(-COOH yetheminali C | I-KLH(-NH2 ye-N itheminali) | I-OVA(-COOH yetheminali C) |
| I-BSA Conjugation ku-Cys | Ukuhlanganiswa kwe-KLH ku-Cys | I-OVA(-NH2 ye-N terminal) |
| Ukuhlanganiswa kwe-OVA ku-Cys |
I-Phosphorylation
| {pSer} | {D-pSer} |
| {pTyr} | {D-pTyr} |
| {pThr} | {D-pThr} |
Okunye ukuguqulwa(PEGylation, Cyclic modifications,Disulfide Bridges)
| I-PEGylation | Ukuguqulwa kwamabhayisikili | I-Disulfide Bridges |
| {Mini-PEG1} | Hamba uye emsileni umjikelezo | {Ibhuloho le-Mono Disulfide} |
| {Mini-PEG2} | {Ibhuloho le-Double Disulfide} | |
| {PEG-11} | {Ibhuloho le-Triple Disulfide} | |
| {PEG-12} | {Ibhuloho le-Disulfide elingahleliwe} | |
| …… | …… | …… |