Ukuhlaziywa kwezindlela zokuhlanganisa ezifana ne-peptide

Ubuchwepheshe bokuhlanganisa obufana ne-peptide

Ucwaningo nokuthuthukiswa kwezidakamizwa ze-peptide lukhula ngokushesha kwezokwelapha.Kodwa-ke, ukuthuthukiswa kwezidakamizwa ze-peptide kunqunyelwe izici zabo siqu.Isibonelo, ngenxa yokuzwela okukhethekile kwe-enzymatic hydrolysis, ukuzinza kuyancishiswa, futhi ukuhlukahluka kwe-steric conformation kubangela ukucaciswa okuhlosiwe okuphansi, i-hydrophobicity ephansi, nokuntuleka kwesistimu ethile yokuthutha.Ukuze unqobe lawa ma-peptide, izixazululo eziningi ezihlongozwayo nokusebenzisa ngempumelelo uhlobo olulodwa lwe-peptide kungenye yazo.

Uhlobo lwe-peptide (igama lesiNgisi: Peptoid) noma i-Poly – N – esikhundleni se-glycine (igama lesiNgisi: I-Poly real – N – substitutedglycine), iyinhlanganisela ye-quasi peptide ye-peptide eketangoni elikhulu.Iketango le-alpha carbon side lidlulisa iketango elikhulu le-nitrogen esikhundleni seketango elisemaceleni.Ku-polypeptide yasekuqaleni, iqembu le-R le-amino acid side chain limelela ama-amino acid angama-20 ahlukene, kodwa iqembu le-R linezinketho eziningi ku-peptoid.Ku-peptide, i-peptide ochungechungeni oluyinhloko lwama-amino acid ku-alpha carbon nitrogen esikhundleni sokudluliswa kweketango elisemaceleni liye eketangoni elikhulu.Kuyafaneleka ukusho ukuthi ama-peptide ngokuvamile awakhiqizi izakhiwo ezi-oda eziphezulu ezifana nezakhiwo zesibili kuma-peptide namaprotheni ngenxa yokuntuleka kwe-hydrogen ku-nitrogen yomgogodla.Inhloso yokuqala ye-Peptide ukwakha i-peptide eqinile ne-protease yezidakamizwa ezincane ze-molecule.

Ukuhlaziywa kwezindlela zokuhlanganisa ezifana ne-peptide

Indlela ye-peptide synthesis yethulwa

Indlela yokuhlanganisa efana ne-peptide evame ukuthandwa iyindlela yokuhlanganisa eyodwa eyasungulwa uRonZuckermann, ngayinye ehlukaniswe ngezinyathelo ezimbili: i-acylation kanye nokugudluzwa.Ku-acylation, isinyathelo sokuqala ukwenza kusebenze i-haloacetic acid ukuze isabele nama-amine asele ekupheleni kwesinyathelo sangaphambilini, ngokuvamile i-disopropyl carbonized diimine.I-bromoacetic acid yenziwe yasebenza nge-disopropylcarbodiimide."Ekuphenduleni kokubuyisela (i-bimolecular nucleophilic substitution reactions), i-amine, ngokuvamile eyinhloko, ihlasela enye i-halogen ukuze yakhe i-glycine efakwe esikhundleni se-N."Umzila we-subunitary synthetic usebenzisa ama-amine ayinhloko atholakala kalula ukuze ukhiqize ama-peptide, ngaleyo ndlela uvumela ukuhlanganiswa kwamakhemikhali kwama-peptide.

Isandiso esiqinile ekuhlanganiseni kwama-peptide ekilasini sinolwazi olucebile, singakunikeza uhlobo lwesevisi ye-peptide synthesis.

Ukuhlaziywa kwezindlela zokuhlanganisa ezifana ne-peptide

Inzuzo ye-peptide enjalo

Izinzile: ama-peptoid azinzile ku-vivo kunama-peptide.

Ukukhetha okwengeziwe: Ama-Peptoid afaneleka kahle ezifundweni zokutholwa kwezidakamizwa ezihlanganisiwe ngoba izinhlobonhlobo ezinkulu zamabhulokhi wokwakha we-polypeptide zingatholwa ngokuguqulwa kweqembu le-amino lomgogodla.

Isebenza kahle kakhulu: Ubuningi bezakhiwo ze-peptoid kungenza i-peptoid ibe yisinqumo esihle sendlela yokuskena ukuze ithole ngokushesha izakhiwo ezithile ezibophezela amaprotheni.

Amandla emakethe engeziwe: izici zohlobo lwe-peptide makube uhlobo lokuthuthukiswa kwezidakamizwa olunamandla amakhulu.