Isikimu sokuklama nesisombululo seketango le-polypeptide peptide

I. Isifinyezo
Ama-peptides ama-macromolecules akhethekile kangangokuthi ukulandelana kwawo akujwayelekile ezakhini zawo zamakhemikhali nezomzimba.Amanye ama-peptide anzima ukuwahlanganisa, kuyilapho amanye kulula ukuwahlanganisa kodwa kunzima ukuwahlanza.Inkinga engokoqobo ukuthi ama-peptides amaningi ancibilika kancane ezixazululweni ezinamanzi, ngakho-ke ekuhlanzeni kwethu, ingxenye ehambisanayo ye-peptide ye-hydrophobic kumele incibilikiswe kumakhemikhali angenawo amanzi, Ngakho-ke, lezi zincibilikizi noma ama-buffers kungenzeka ukuthi angahambisani kakhulu nokusetshenziswa. zezinqubo zokuhlola zebhayoloji, ukuze ochwepheshe bavinjelwe ngokuphelele ukusebenzisa i-peptide ngezinhloso zabo, ukuze okulandelayo kube yizici ezimbalwa zokwakheka kwama-peptide kubacwaningi.

Isikimu sokuklama nesisombululo seketango le-polypeptide peptide
Okwesibili, ukukhetha okulungile kwama-peptide anzima okwenziwa
1. Ubude obuphelele bokulandelana okulawulwa phansi
Ama-peptide angaphansi kwezinsalela eziyi-15 kulula ukuwathola ngoba ubukhulu be-peptide buyanda futhi ubumsulwa bomkhiqizo ongahluziwe buyancipha.Njengoba ubude obuphelele bochungechunge lwe-peptide bukhuphuka ngaphezu kwezinsalela ezingama-20, inani eliqondile lomkhiqizo liwukukhathazeka okubalulekile.Ezivivinyweni eziningi, kulula ukuthola imiphumela engalindelekile ngokwehlisa inombolo yensalela ngaphansi kuka-20.
2. Yehlisa inani lezinsalela ze-hydrophobic
Ama-Peptides anenqwaba yezinsalela ze-hydrophobic, ikakhulukazi esifundeni 7-12 izinsalela ezivela ku-C-terminus, ngokuvamile abangela ubunzima bokwenziwa.Lokhu kubonakala njengenhlanganisela enganele ngenxa yokuthi ishidi le-B-fold litholakala ekuhlanganiseni."Ezimweni ezinjalo, kungase kube usizo ukuguqula izinsalela ezingaphezu kwezimbili ezinhle nezingezinhle, noma ukufaka i-Gly noma i-Pro ku-peptide ukuze uvule ukwakheka kwe-peptide."
3. Ukulawulwa phansi kwezinsalela “ezinzima”
"Kunenqwaba yezinsalela ze-Cys, Met, Arg, kanye ne-Try ngokuvamile ezingahlanganiswa kalula."I-Ser ngokuvamile izosetshenziswa njengenye indlela ye-nonoxidative ku-Cys.
Isikimu sokuklama nesisombululo seketango le-polypeptide peptide

Okwesithathu, thuthukisa ukukhetha okulungile kokuncibilika emanzini
1. Lungisa itheminali engu-N noma C
Ngokuphathelene nama-peptide ane-acidic (okungukuthi, akhokhiswe kabi ku-pH 7), i-acetylation (i-N-terminus acetylation, i-C terminus ehlala igcina iqembu lamahhala le-carboxyl) iyanconywa ikakhulukazi ukukhulisa inkokhiso engemihle.Kodwa-ke, kuma-peptide ayisisekelo (okungukuthi, akhokhiswe kahle ku-pH 7), ukukhishwa (iqembu lamahhala le-amino ku-N-terminus kanye nokugcotshwa ku-C-terminus) kunconywa ikakhulukazi ukukhulisa inkokhiso enhle.

2. Fushanisa kakhulu noma andise ukulandelana

Okunye ukulandelana kuqukethe inani elikhulu lama-amino acid e-hydrophobic, njenge-Trp, Phe, Val, Ile, Leu, Met, Tyr kanye ne-Ala, njll. Uma lezi zinsalela ze-hydrophobic zidlula i-50%, ngokuvamile akulula ukuhlakazeka.Kungase kube usizo ukwelula ukulandelana ukuze kuthuthukiswe izigxobo ezinhle nezingezinhle ze-peptide.Inketho yesibili ukwehlisa usayizi weketango le-peptide ukuze kwandiswe izigxobo ezinhle nezingezinhle ngokunciphisa izinsalela ze-hydrophobic.Uma izinhlangothi ezinhle nezingezinhle zochungechunge lwe-peptide zinamandla, maningi amathuba okuba zisabele ngamanzi.
3. Faka insalela encibilika emanzini
Kwamanye amaketanga e-peptide, inhlanganisela yamanye ama-amino acid amahle kanye namabi angathuthukisa ukunyibilika kwamanzi.Inkampani yethu incoma i-N-terminus noma i-C-terminus yama-peptide ane-asidi ukuthi ihlanganiswe ne-Glu-Glu.I-terminus N noma C ye-peptide eyisisekelo yanikezwa kwase kuba yi-Lys-Ls.Uma iqembu elishajiwe lingakwazi ukubekwa, i-Ser-Gly-Ser nayo ingafakwa ku-N noma C terminus.Kodwa-ke, le ndlela ayisebenzi lapho izinhlangothi zeketango le-peptide zingenakushintshwa.