Ngesikhathi sokuhlanganiswa kwe-peptide, omunye usawoti udinga ukungezwa.Kodwa kunezinhlobo eziningi zikasawoti, futhi izinhlobo ezahlukene zikasawoti zenza ama-peptide ahlukene, futhi umphumela awufani.Ngakho-ke namuhla sikhetha ikakhulukazi uhlobo olufanele lukasawoti we-peptide ku-peptide synthesis.
1. I-Trifluoroacetate (TFA) : Lona usawoti ovame ukusetshenziswa emikhiqizweni ye-peptide, kodwa udinga ukugwenywa kwezinye izivivinyo ngenxa ye-biotoxicity ye-trifluoroacetate.Isibonelo, ukuhlolwa kweseli.
2. I-Acetate (AC) : I-biotoxicity ye-acetic acid incane kakhulu kune-trifluoroacetic acid, ngakho-ke ama-peptide amaningi emithi nezimonyo asebenzisa i-acetate, kodwa eminye imikhiqizo ine-acetate engazinzile, ngakho ukuzinza kokulandelana nakho kudinga ukucatshangelwa.I-acetate ikhethelwe ukuhlola okuningi kweseli.
3. I-Hydrochloric acid (HCL) : Lo sawoti awuvamile ukukhethwa, futhi okunye ukulandelana kuphela kusebenzisa i-hydrochloric acid ngezinjongo ezikhethekile.
4. Usawoti we-Ammonium (NH4+) : Lo sawoti uzophazamisa kakhulu ukuncibilika nokuzinza komkhiqizo, kufanele ukhethwe ngokulandelana kwawo.
5. Usawoti we-sodium (NA+) : ngokuvamile uthinta ukuzinza nokuncibilika komkhiqizo.
6. I-Pamoicacid: Lo sawoti uvame ukusetshenziswa emithini ye-peptide ukwenza ama-ejenti akhulula isikhathi eside.
7. I-CitricAcid: Lo sawoti unobuthi obuncane bomzimba, kodwa ukulungiswa kwawo kuyinkimbinkimbi kakhulu, ngakho-ke inqubo yokukhiqiza idinga ukuthuthukiswa ngokulandelana nangokuhlukana.
8. I-Salicylicacid: I-Salicylate ingathinta ukuzinza kwemikhiqizo ye-peptide, ngakho ayivamile ukusetshenziswa.
Okungenhla kukhona izinhlobo eziningana zikasawoti we-peptide, futhi kufanele sikhethe ngokuvumelana nezici zosawoti abahlukene ekusetshenzisweni kwangempela.
Isikhathi sokuthumela: Jun-16-2023