Ngokwendlela yokuxhuma ye-amino acid noshukela, i-peptide kashukela ingahlukaniswa ngezigaba ezine: O glycosylation, C a N glycosylation, dew saccharification kanye ne-GPI (glycophosphatidlyinositol) uxhumano.
1. I-N-glycosylation glycopeptides yenziwe ngeglucose ye-N-acetamide ekugcineni okwehliswayo kweketango le-glycan (Glc-Nac) elixhunywe ku-athomu engu-N eqenjini le-amide leketango eliseceleni le-Asn ethile kuketango le-peptide, kanye ne-Asn. ekwazi ukuxhumanisa iketango le-glycan kufanele ibekwe ku-AsN-X-Ser /Thr (X! =P) ku-motif eyakhiwe yizinsalela.Ushukela yi-N-acetylglucosamine.
I-N-glycosylation modified structural glycopeptide
2. Isakhiwo se-O-glycosylation silula kune-N-glycosylation.Le glycopeptide ivamise ukuba mfishane kune-glycan, kodwa inezinhlobo eziningi kune-N-glycosylation.I-Ser ne-Thr ngokuvamile zingafakwa i-glycosylated ochungechungeni lwe-peptide.Ngaphezu kwalokho, kukhona ama-glycopeptides ahlotshiswe nge-tyrosine, i-hydroxyl, ne-hydroxyproline glycosylation.Indawo yokuxhumanisa i-athomu ye-oksijini ye-hydroxyl esochungechungeni lwezinsalela.Ushukela oxhunyiwe yi-galactose noma i-N-acetylgalactosamine (Gal&GalNAc) noma i-glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), njll.
I-O-glycosylation ishintsha isakhiwo
3. I-Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
I-N-acetylglucosamine (GlcNAc) glycosylation eyodwa ixhuma amaprotheni i-O-GlcNAc ku-athomu ye-hydroxyl oxygen ye-serine noma insalela ye-threonine yephrotheni.I-O-GlcNA glycosylation i-O-GlcNAc monosaccharide umhlobiso ngaphandle kwesandiso se-glycan;Njenge-peptide phosphorylation, i-O-GlcNAc glycosylation ye-glycopeptides futhi iyinqubo yokuhlobisa amaprotheni ashukumisayo.Ukuhlobisa okungavamile kwe-O-GlcNAc kungabangela izifo ezihlukahlukene ezifana nesifo sikashukela, izifo zenhliziyo, izimila, isifo se-Alzheimer nokunye.
Amaphuzu we-glycosylation we-glycopeptides
Izakhiwo eziyisisekelo ze-polypeptide namaketanga kashukela zixhunyaniswa namaketanga amaprotheni ngamabhondi ahlangene, futhi amasayithi axhumanisa amaketanga kashukela abizwa ngokuthi amasayithi e-glycosylation.Njengoba kungekho isifanekiso sokulandela i-biosynthesis yamaketanga kashukela e-glycopeptide, amaketanga ahlukene kashukela azoxhunywa endaweni efanayo ye-glycosylation, okuholela kulokho okubizwa ngokuthi i-microscopic inhomogeneity.
I-glycosylation ye-glycopeptides
1. Umphumela we-glycopeptide glycosylation ekwelapheni-ukuphumelela kwamaprotheni okwelapha
Endabeni yamaprotheni e-therapy-therapeutic, i-glycosylation iphinde ithinte uhhafu wempilo kanye nokukhomba izidakamizwa zamaprotheni ku-vivo.
2. I-glycopeptide glycosylation encibilikayo namaprotheni
Ucwaningo luye lwabonisa ukuthi amaketanga kashukela ebusweni bamaprotheni angathuthukisa ukuncibilika kwamangqamuzana amaprotheni
3. I-Glycopeptide glycosylation kanye ne-protein immunogenicity
Ngakolunye uhlangothi, amaketanga kashukela ebusweni bamaprotheni angabangela izimpendulo ezithile zokuzivikela komzimba.Ngakolunye uhlangothi, amaketanga kashukela angakwazi ukumboza izindawo ezithile endaweni yamaprotheni futhi anciphise ukuzivikela kwawo
4. I-Glycopeptide glycosylation eyandisa ukuqina kwamaprotheni
I-Glycosylation ingakhuphula ukuzinza kwamaprotheni ezimeni ezihlukahlukene zokuguquguquka (njengama-denaturation, ukushisa, njll.) futhi igweme ukuhlangana kwamaprotheni.Ngasikhathi sinye, amaketanga kashukela angaphezulu kwamaprotheni angaphinda amboze amaphuzu athile okuwohloka kwe-proteolytic yama-molecule wamaprotheni, ngaleyo ndlela andise ukumelana kwamaprotheni kuma-protein.
5. I-Glycopeptide glycosylation ethinta umsebenzi webhayoloji wama-molecule wamaprotheni
Ukushintsha i-protein glycosylation nakho kungenza ama-molecule wamaprotheni enze imisebenzi emisha yebhayoloji
Isikhathi sokuthumela: Aug-03-2023