Indlela entsha yokwenza kanye nokulungiswa kwe-thymectomy

Indlela entsha ye-Thymus yakhiwe nge-28 amino acid synthetic polypeptide immune regulator.Indlela ye-Thymus iwuhlobo lwemithi emisha ye-uct, evame ukusetshenziswa ezigulini zesimila, ingathuthukisa amasosha omzimba.

Indlela yokwenza

Indlela eqondile ye-thymalfasin ekwelapheni i-hepatitis B engapheli ayizange ifundwe ngokugcwele, kodwa ukuhlolwa okuhlukahlukene kuye kwabonisa ukuthi i-thymalfasin inokusabela okuphawulekayo komzimba.Ifezwa ngokuyinhloko ngokuthuthukisa ukusebenza kwe-T cell, futhi idlulela ezifundazweni ezibandakanya ukukhiqizwa kwamaseli e-T, ukuhlukaniswa nokucwengwa, kanye ne-apoptosis.Ngokwandisa inani eliphelele le-lymphoid, umbulali wemvelo we-intrahepatic, amaseli e-CD3 nama-CD4, i-thymalfasin ingadlala indima yokuzivikela komzimba.I-Pleural fasin ingakhuthaza ukuvuthwa nokuhlukaniswa kwamaseli e-DC, futhi ngaleyo ndlela ikhuthaze ukuvuthwa nokuhlukaniswa kwamaseli e-T.I-Thymalfasin ingakwazi ukusebenzisa i-mitogen noma i-antigen, ithuthukise ukukhiqizwa kwezinhlobonhlobo zama-cytokine njenge-interferon human interleukin IL2 kanye ne-IL3, ithuthukise ukuvezwa kwe-interleukin-2 receptor, futhi yenze amaseli e-CD4T ahluke abe yi-THLS, enemiphumela ebalulekile yokulwa namagciwane.Ngokwandisa indima yama-P cell, ingakhuthaza ukukhiqizwa kwama-lymphocyte ku-vivo, ilungise ingxenye yama-P cell subsets kanye nokuhlukaniswa kwamaseli e-U, kanye nokukhiqizwa kwamasosha omzimba, ikhuthaze ukukhiqizwa kwamanye ama-lymphokines alwa namagciwane nama-macrophage, ithuthukise ukugwinya. isebenze, futhi ithuthukise umsebenzi wamasosha omzimba eziguli ezinomdlavuza.Ingakwazi futhi ukususa amagciwane, amabhaktheriya kanye namagciwane emzimbeni.Incwadi ye-Thymus yesistimu yezinzwa kanye ne-endothelium ezungezile, iphakama ukuze ilungise indima yokudluliselwa kwe-hippocampal neuron synaptic.Indlela ye-Thymus entsha yokudluliswa kwe-synaptic ejabulisayo kanye nesistimu yezinzwa, ingasetshenziswa ukuvimbela iziguli ezinomdlavuza obangelwa ukwelashwa komdlavuza okufana ne-chemotherapy kanye ne-neurotoxicity.I-Pleuraxin inemiphumela yokulawula izakhi zofuzo, ingaheha izakhi zofuzo ze-kinase, ikhulise izakhi zofuzo ze-cytokine, izakhi zofuzo ezilawula ukusabela komzimba.Ukulawulwa okuphezulu kwe-thymalfasin kuzakhi zofuzo zesamukeli ezifana ne-IL kuphakamisa ukuthi i-thymalfasin ivuselela amaseli ezethulo we-antigen."Ngaphezu kwalokho, umphumela we-thymalfasin ekukhuleni kwamangqamuzana omzimba omzimba ubonakala ekwandeni kwezici zokukhula kwe-stem cell kanye nezakhi zofuzo ze-hematopoietic protein."

Ukulandelana okusha kwe-thymectomy

Inqubo yokulungiselela

1. Indlela yokukhipha amakhemikhali ezinto eziphilayo Amalungiselelo e-Thymosin (ingxube yama-peptide) atholakala ezicutshini zezilwane, ngakho-ke umsebenzi uhluke kakhulu.Ngenxa yokuthi kakade iqukethe amaprotheni ezilwane, maningi amathuba okuba nezimpawu zokungezwani komzimba.Indlela etholwe ngamakhemikhali e-thymus evela ku-thymus ye-embryonic yabantu yintsha, inesivuno esincane kakhulu kodwa isebenza kahle kakhulu.Uma kucatshangelwa izinto zokusetshenziswa ezilinganiselwe, intengo ephezulu, isivuno esiphansi, kanye nenqubo yokutholwa eyinkimbinkimbi., ubunzima bokukhiqiza obukhulu, ithemba lentuthuko alinalo ithemba.

2. Ukwenziwa kwamakhemikhali I-thymusfasin etholakala ngokwezentengiso ikhiqizwa ikakhulukazi njenge-SPSS, ekhiqizwa futhi ihlanzwe ngamasu afanele.Ngokusekelwe ku-resin, i-Fmoc yasetshenziselwa ukwakheka kwesigaba esiqinile, futhi izinhlobo ezingama-28 zama-amino acid zakhiqizwa ngokuya nge-thymalfasin ngokulandelana.Ama-amino acid e-N-terminal ayenziwe i-acetylated ne-acetic anhydride, futhi i-TFA yasetshenziswa njenge-reagent ebalulekile yokuqhekeka ukuze kutholakale imikhiqizo enzima.Umkhiqizo ongahluziwe wahlukaniswa yi-HPLC ukuze kutholwe umkhiqizo omusha wendlela ye-thymus, owabuye wacwengwa ukuze kutholwe indlela entsha ye-thymus impahla eluhlaza.

Kunezinhlobo ezintathu zamabhaktheriya akhiwe ngofuzo1) Izakhi zofuzo eziningi zihlanganiswa kumsingathi ukuze kutholwe izakhi zofuzo eziqondiwe, futhi ekugcineni zivezwe ngendlela yemizimba efakiwe.Kubikwe ukuthi izakhi zofuzo ezinezintambo eziningi zenoveli ye-thymmethod zasungulwa ngamasu okukhipha okungahleliwe kanye ne-PCR futhi aphindaphindwa ku-Escherichia coli.(2) Isakhi sofuzo sephrotheni esihlanganisiwe saphinde sahlanganiswa kwabezindaba, futhi abezindaba baguqulwa baba umsingathi ukuze akhe amagciwane akhiwe ngofuzo.Isibonelo, ngokusekelwe esakhini se-peptide yesignali ku-plasmid, inkulumo ethi plasmid yakhiwa, yaguqulwa yaba i-Pichia pastoris nge-electroexcitation, bese iphrotheni ye-fusion yenziwa yi-methanol, bese indlela entsha ye-thymus yakhiwa ngokugayeka kwe-enzyme nezinye izindlela. .