Ama-antigen amaprotheni aphinde abe nama-epitopes amaningana ahlukene, amanye awo ama-epitopes alandelanayo kanti amanye angama-epitopes esakhiwo.Amasosha omzimba e-Polyclonal atholwe ngokugoma izilwane ngama-antigens enziwe yi-denatured ayingxube yamasosha omzimba aqondene nama-epitopi angawodwana futhi angasetshenziswa ngokujwayelekile ukuthola izakhiwo zemvelo noma amaprotheni ahlosiwe.Inzuzo eseceleni yokusebenzisa amaprotheni angama-denatured njengama-immunogens ukuthi amaprotheni angama-denatured avame ukuba ne-immunogenic futhi angavuselela ukusabela okuqinile kwamasosha omzimba ezilwaneni.
Uhlelo lokukhuluma lwe-Escherichia coli luvamise ukukhethwa ngokwezinjongo ze-antigenic ngoba luwuhlelo olubiza kakhulu ngokwesikhathi nemali.Ukuze kuthuthukiswe ukuthi kungenzeka yini ukuvezwa kwephrotheni okuhlosiwe kanye nokuba lula kokuhlanzwa, ngezinye izikhathi kuphela ingxenye encane yeprotheni eqondiwe, njengesizinda esithile, esivezwayo.
Isakhiwo esinezinhlangothi ezintathu samaprotheni
Isizinda Esiqondile Sephrotheni
Uma inhloso yokulungiswa kwe-antibody iwukuthola i-wb kuphela, kuyonga futhi kuyashesha ukusebenzisa i-peptide encane yokwenziwa njenge-antigen, kodwa kunengozi yokuzivikela komzimba okubuthakathaka noma ukungaphindiseli ngenxa yokukhethwa okungalungile kwengxenye ye-peptide.Njengoba ukulungiswa kwe-antibody kudinga isikhathi eside, izingxenye ezimbili noma ezintathu ezahlukene ze-peptide zivame ukukhethwa ukuze kulungiswe amasosha omzimba kusetshenziswa i-polypeptide antigen ukuze kuqinisekiswe izinga lokuphumelela lokuhlolwa.
Ukuhlanzeka kwe-antigen ye-polypeptide yokugoma kuyadingeka ukuthi kube ngaphezu kwama-80%.Nakuba ukuhlanzeka okuphezulu kungathola amasosha omzimba ngokucaciswa okungcono, ngokusebenza, izilwane zihlala zikhiqiza inani elikhulu lamasosha omzimba angaqondile, ngaleyo ndlela zifihla izinzuzo zokuhlanzeka kwe-antigen.
Ukwengeza, ukulungiswa kwamasosha omzimba avela kuma-peptide amancane kufanele kuxhunyaniswe ne-antigen eyisithwali efanelekile ukuze kuthuthukiswe ukuzivikela kwayo.Izithwali ezimbili ezijwayelekile ze-antigenic yi-KLH ne-BSA.
Isikhathi sokuthumela: Mar-23-2023