Ayini ama-peptide angena kumaseli?

Ama-peptide angena kumaseli angama-peptide amancane angangena kalula kulwelwesi lwamaseli.Leli klasi lama-molecule, ikakhulukazi ama-CPP anemisebenzi yokukhomba, linesithembiso sokulethwa kwezidakamizwa okusebenzayo kumaseli okuqondiwe.

Ngakho-ke, ucwaningo olukulo lunokubaluleka okuthile kwe-biomedical.Kulolu cwaningo, ama-CPP anemisebenzi ehlukene ye-transmembrane afundwe ezingeni lokulandelana, ezama ukuthola izici ezithinta umsebenzi we-transmembrane we-CPPs, umehluko wokulandelana phakathi kwama-CPP anemisebenzi ehlukene kanye nama-NonCPP, futhi wethula indlela yokuhlaziya ukulandelana kwezinto eziphilayo.

Ama-CPP kanye nama-NonCPP alandelanayo atholwe kusizindalwazi se-CPPsite kanye nezincwadi ezihlukene, kanye nama-transmembrane peptides (ama-HCPP, ama-MCPP, ama-LCPP) anomsebenzi we-transmembrane ophezulu, omaphakathi, nophansi akhishwe ekulandeleni kwe-CPP ukuze kwakhiwe amasethi edatha.Ngokusekelwe kulawa masethi edatha, kwenziwa izifundo ezilandelayo:

I-1, I-amino acid kanye nokwakheka kwesakhiwo sesibili se-CPP ehlukene esebenzayo kanye nama-NonCPPs kwahlaziywa yi-ANOVA.Kutholwe ukuthi ukusebenzisana kwe-electrostatic kanye ne-hydrophobic kwama-amino acid kudlale indima ebalulekile emsebenzini we-transmembrane wama-CPP, futhi ukwakheka kwe-helical nokugoqa okungahleliwe nakho kuthinte umsebenzi we-transmembrane wama-CPP.

2. Izinto ezibonakalayo namakhemikhali kanye nobude bama-CPP anemisebenzi ehlukene kuboniswe endizeni enezinhlangothi ezimbili.Kutholakale ukuthi ama-CPP kanye nama-NonCPP anemisebenzi ehlukene angaqoqwa ngaphansi kwezakhiwo ezithile ezikhethekile, futhi ama-HCPP, ama-MCPP, ama-LCPP nama-NonCPP ahlukaniswe amaqoqo amathathu, abonisa ukuhlukana kwawo;

3. Kuleli phepha, umqondo we-centroid ngokomzimba namakhemikhali wokulandelana kwebhayoloji wethulwa, futhi izinsalela ezihlanganisa ukulandelana zibhekwa njengamaphoyinti ezinhlayiya, futhi ukulandelana kukhishwe njengohlelo lwezinhlayiyana zocwaningo.Le ndlela yasetshenziswa ekuhlaziyeni ama-CPP ngokuveza ama-CPP anemisebenzi ehlukene endizeni ye-3D ngendlela ye-PCA, futhi kwatholakala ukuthi ama-CPP amaningi ahlanganiswe ndawonye futhi amanye ama-LCPP ahlanganiswe ndawonye nama-NonCPP.

Lolu cwaningo lunomthelela ekwakhiweni kwama-CPP nokuqonda umehluko wokulandelana kwama-CPP nemisebenzi ehlukene.Ukwengeza, indlela yokuhlaziya ye-centroid yomzimba nekhemikhali yokulandelana kwebhayoloji eyethulwe kuleli phepha ingase isetshenziselwe ukuhlaziya ezinye izinkinga zebhayoloji.Ngesikhathi esifanayo, angasetshenziswa njengamapharamitha okokufaka wezinkinga ezithile zokuhlukaniswa kwebhayoloji futhi adlale indima ekubonweni kwephethini.