Uyini umumo wama-peptide enziwe ngokwezifiso?Lawa maphuzu uyawazi?

I-Peptide chain synthesis isetshenziswe kabanzi emikhakheni eminingi, ikakhulukazi ekuthuthukisweni kwezidakamizwa, ucwaningo lwebhayoloji kanye ne-biotechnology.Ama-Peptide anobude obuhlukahlukene nokulandelana angahlanganiswa ngokusebenzisa i-peptide chain synthesis yokulungiselela izidakamizwa, umthwali wezidakamizwa, ukuhlaziya amaprotheni, ucwaningo olusebenzayo nokunye.Ngakho-ke isiphi isiqondiso sama-peptides okwenziwa?Namuhla, uGutuo Xiaobian uzokunikeza impendulo enemininingwane ngezansi.

Uyini umumo wama-peptide enziwe ngokwezifiso?Uyawazi la maphuzu?

I-Peptide chain synthesis ingafinyelelwa ngokungezwa okuhamba kancane kwama-amino acid molecule eketango elikhona le-peptide, okukhiqiza amabhondi amasha e-peptide.Ngenxa yokukhula kusukela ekupheleni kuka-N kuya ku-C peptide chain, ngakho-ke isiqondiso se-synthesis futhi sisuka ekupheleni kuka-N kuya ku-C. Lokhu kungenxa yokuthi i-C terminal kanye ne-N terminal amino acids phakathi kwenqubo yokwakhiwa kwebhondi ye-peptide, ama-amino acids of carboxyl end. (C) kanye ne-amino ekhona (N) ekugcineni kokusabela kweketango le-peptide, kubangela ibhondi entsha ye-peptide.Ngakho-ke, isiqondiso se-synthesis kusukela ekupheleni kuka-N kuya ku-C.

I-Peptide synthesis ngokuyinhloko ixhuma ama-amino acid molecule ngokusebenzisa indlela yamakhemikhali ukwakha inqubo yama-peptide.Ukucacisa, i-polypeptide chain synthesis ixhuma ama-amino acid molecule, yona, ingakhiwa ngamabhondi amakhemikhali ukuze kwakhiwe inqubo yama-peptide.I-Peptide chain synthesis ngokuvamile ingenziwa ngokuhlanganisa isigaba esiqinile noma ukuhlanganiswa kwesigaba se-liquid.Ku-solid-phase synthesis, ama-amino acid okuqala anamathiselwe ku-solid-phase material, bese kuthi iketango le-peptide linwetshwe ngokulandelana ngokungezwa kwe-amino acid ngayinye.

Ngenhla i-make up encane yokwethula ukuhlukaniswa kwe-peptide kanye nendima yolwazi oluhlobene, i-tinto eqinile igxile ekuhlanganiseni kwe-peptide, ukubonakaliswa kwamaprotheni, ukulungiswa kwe-antibody, njll., ihileleke embonini ye-peptide iminyaka eminingi, ikhwalithi yomkhiqizo wezinga lokuqala , wamukele izindimbane zamakhasimende azobonisana.