I-Suc-Leu-Leu-Val-Tyr-AMC/94367-21-2/GT Peptide/Peptide Supplier

Incazelo 

I-Suc-Leu-Leu-Val-Tyr-AMCi-substrate ye-fluorescent.I-Huc-leu-leu-val-tyr-amc (Huc-LLVY) iyi-membrane-permeable calpain-specific fluorescent substrate, futhi i-proteolysis yamabhondi e-peptidyl-7-amino ikhulula i-fluorescent ephezulu i-7-amino-4-methylcoumarin (AMC) ingxenye.Umphumela we-TGF-β ku-hydrolysis yalawa ma-substrates, isibonelo, i-huc-Leu-Leu-Val-Tyr-AMC, yahlolwa.Amaseli e-bile duct epithelial H69 afakwe ku-10, 1, 0.1, noma 0 ng/mL TGF-β amahora angama-24.I-substrate hydrolysis ku-cytosolic extracts yabe ihlolwa nge-fluorometry.Imisebenzi eyisisekelo ye-hydrolysis ye-HUC-LLVY-AMC, z-LLE-AMC, ne-z-LLL-AMC yayingu-1.12, 8.33, kanye ne-14.52 nmol AMC/mg amaprotheni/min, ngokulandelana.

Imininingwane

Ukubukeka: Impushana emhlophe ukuya kokumhlophe

Ubumsulwa(HPLC):≥98.0%

Ukungcola Okukodwa:≤2.0%

Okuqukethwe kwe-Acetate(HPLC): 5.0%～12.0%

Okuqukethwe Amanzi (Karl Fischer):≤10.0%

Okuqukethwe kwe-Peptide:≥80.0%

Ukupakisha Nokuthumela: Izinga lokushisa eliphansi, ukupakisha kwe-vacuum, kunembile ku-mg njengoba kudingeka.

Unga-oda Kanjani?

1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.

2. Oda ku-inthanethi.Sicela ugcwalise ifomu le-oda ku-inthanethi.

3. Nikeza igama le-peptide, Inombolo ye-CAS noma ukulandelana, ukuhlanzeka nokuguqulwa uma kudingeka, ubuningi, njll. sizohlinzeka ngekhotheshini kungakapheli amahora ama-2.

4. Ukugcinwa kwe-oda ngenkontileka yokuthengisa esayiniwe ngokufanelekile kanye ne-NDA(isivumelwano esingadalulwanga) noma isivumelwano esiyimfihlo.

5. Sizoqhubeka nokubuyekeza inqubekelaphambili ye-oda ngesikhathi.

6. Ukulethwa kwe-Peptide nge-DHL, i-Fedex noma abanye, kanye ne-HPLC, MS, COA izohlinzekwa kanye nempahla.

7. Inqubomgomo yembuyiselo izolandelwa uma kukhona ukushayisana kwekhwalithi noma isevisi yethu.

8. Isevisi yangemuva kokuthengisa: Uma amakhasimende ethu enemibuzo mayelana ne-peptide yethu ngesikhathi sokuhlolwa, sicela ukhululeke ukusithinta futhi sizoyiphendula ngokushesha.

Yonke imikhiqizo yenkampani isetshenziselwa inhloso yocwaningo lwesayensi kuphela, yona'kwenqatshelwe ukusetshenziswa ngokuqondile yinoma yimuphi umuntu emzimbeni womuntu.

FAQ:

Ingabe ama-peptide aqukethe ama-Cys ancishisiwe ngaphambi kokuthunyelwa?

Uma i-peptide ingatholakali ukuthi ifakwe i-oxidized, ngokuvamile asinciphisi ama-Cys.Wonke ama-polypeptide atholakala emikhiqizweni engcolile ehlanjululwe futhi i-lyophilized ngaphansi kwezimo ze-pH2, okuthi okungenani ngokwezinga elithile ivimbele ukukhishwa kwe-Cys.Ama-Peptide aqukethe ama-Cys ahlanzwa ku-pH2 ngaphandle uma kunesizathu esithile sokuhlanza ku-pH6.8.Uma ukuhlanzwa kwenziwa ku-pH6.8, umkhiqizo ohlanziwe kufanele uphathwe nge-asidi ngokushesha ukuze uvimbele ukugcotshwa kwe-oxidation.Esinyathelweni sokugcina sokulawula ikhwalithi, kuma-peptide aqukethe ama-Cys, uma ubukhona besisindo samangqamuzana (2P+H) butholakala kumephu ye-MS, kukhombisa ukuthi i-dimer yenziwe.Uma ingekho inkinga nge-MS kanye ne-HPLC, sizoziphilisa ngokuqondile futhi sithumele izimpahla ngaphandle kokuqhubeka nokucutshungulwa.Kufanele kuqashelwe ukuthi ama-peptide aqukethe ama-Cys athola i-oxidation ehamba kancane ngokuhamba kwesikhathi, futhi izinga le-oxidation lincike ekulandeleni kwe-peptide nezimo zokugcinwa.

Unquma kanjani ukuthi i-peptide i-looped?

Sisebenzisa ukusabela kuka-Ellman ukuhlola ukuthi ukwakheka kweringi kuqediwe yini.Uma ukuhlolwa kuka-Ellman ku-positive (okuphuzi), ukusabela kwendandatho akuphelele.Uma imiphumela yokuhlolwa inegethivu (hhayi ephuzi), ukusabela kwendandatho sekuqediwe.Asinikezi ngombiko wokuhlaziya wokuhlonza ibhayisikili kumakhasimende ethu.Ngokuvamile, kuzoba nencazelo yemiphumela yokuhlolwa kuka-Ellman embikweni we-QC.

Ngidinga i-cyclic peptide, equkethe i-tryptophan, ingabe izokwenziwa i-oxidized?

I-oxidation ye-tryptophan iyinto evamile ku-peptide oxidation, futhi ama-peptide avame ukuqhutshwa ngamabhayisikili ngaphambi kokuhlanzwa.Uma i-oxidation ye-tryptophan yenzeka, isikhathi sokugcinwa kwe-peptide kukholomu ye-HPLC sizoshintsha, futhi i-oxidation ingasuswa ngokuhlanzwa.Ngaphezu kwalokho, ama-peptide ane-oxidized nawo angatholwa yi-MS.

Ingabe kuyadingeka ukubeka igebe phakathi kwe-peptide nodayi?

Uma uzonamathisela i-molecule enkulu (njengodayi) ku-peptide, kungcono kakhulu ukubeka isikhala phakathi kwe-peptide ne-ligand ukuze unciphise ukuphazamiseka kwesamukeli ngokugoqa i-peptide ngokwayo noma ngokugoqa inhlanganisela yayo.Abanye abazifuni izikhawu.Isibonelo, ekugoqeni amaprotheni, kungenzeka ukunquma ukuthi ukuqhelelana kangakanani isakhiwo esigoqayo se-amino acid ngokunamathisela udayi we-fluorescent endaweni ethile.

Uma ufuna ukwenza ukuguqulwa kwe-biotin kutheminali engu-N, ingabe udinga ukubeka igebe phakathi kwe-biotin nokulandelana kwe-peptide?

Inqubo evamile yokulebula ye-biotin esetshenziswa inkampani yethu ukunamathisela i-Ahx ochungechungeni lwe-peptide, ilandelwe yi-biotin.I-Ahx iyinhlanganisela yekhabhoni engu-6 esebenza njengesithiyo phakathi kwe-peptide ne-biotin.

Ungakwazi yini ukunikeza iseluleko mayelana nokwakhiwa kwama-peptide e-phosphorylated?

Njengoba ubude bukhula, ukusebenza kahle kokubophezela kuyehla kancane kancane kusuka ku-amino acid e-phosphorylated kuye phambili.Isiqondiso se-synthesis sisuka ku-C terminal kuya ku-N terminal.Kutuswa ukuthi izinsalela ngemva kwe-amino acid ene-phosphorylated akumele zidlule ku-10, okungukuthi, inani lezinsalela ze-amino acid ngaphambi kwe-amino acid eyi-phosphorylated esuka ku-N terminal kuya ku-C terminal akufanele idlule ku-10.

Kungani i-n-terminal acetylation kanye ne-C-terminal amidation?

Lezi zinguquko zivimbela i-peptide ekonakalisweni futhi ivumela i-peptide ukuthi ilingise isimo sayo sangempela samaqembu e-alpha amino ne-carboxyl kuphrotheni engumzali.