Ukuguqulwa kwe-methylation

I-Methylation-modified peptides, eyaziwa nangokuthi i-methylation-recognized peptides, ayimihlobiso yangemuva kokuhumusha yamaprotheni (PTMs) futhi idlala indima ebalulekile yokulawula cishe yonke imisebenzi yempilo kumaseli.Amaprotheni acutshungulwa yi-methyltransferase ukuze adlulisele amaqembu e-hydroxyl ezinsalela ezithile ze-amino acid ukuze abophe ngokuhlanganyela.I-Methylation iyinqubo yokuguqula eguqulekayo eyenziwe yi-demethylases.

Ucwaningo luye lwabonisa ukuthi ama-amino acid avamile e-methylated/demethylated ngokuvamile i-lysine (Lys) ne-arginine (Arg).Ucwaningo luye lwathola ukuthi i-histone lysine methylation inemisebenzi ehlukahlukene yezinto eziphilayo ezifana nokugcinwa kwe-stem cell kanye nokuhlukaniswa, ukungasebenzi kwe-X chromosome, ukulawulwa kokubhala kanye nempendulo yokulimala kwe-DNA.", imvamisa ithinta ukufiphala kwe-chromatin futhi icindezela ukwakheka kofuzo."I-Histone arginine methylation idlala indima ebalulekile ekulawuleni ukulotshwa kwezakhi zofuzo futhi ingathinta izinqubo ezihlukahlukene zomzimba kumaseli, okuhlanganisa ukulungiswa kwe-DNA, ukudluliselwa kwesignali, ukuthuthukiswa kwamaseli, kanye ne-carcinogenesis.Ngakho-ke, i-Guopeptide Biology ithuthukise ngokukhethekile ubuchwepheshe be-methyl peptides yokuhlobisa, eguqulwa ososayensi ngemva kokuhumusha amaprotheni (PTMS) ukusiza ocwaningweni.

Ukuguqulwa kwe-Methylation (Me1, Me2, Me3)

Ikhwalithi ephezulu ye-Fmoc-Ls(Me,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) I-2-OH.HCl(asymmetrical), F isetshenziswe i-moc-Arg(me)2-OH.HCl(i-symmetrical) nezinye izinto zokusetshenziswa zenziwe inqubo yokuhlanganiswa kwesigaba esiqinile se-FMOC ukuze kutholwe ama-peptides e-Lys ne-Arg e-methylated, kanye nemikhiqizo. ahlanzwa yi-HPLC.I-mass spectra efanelekile, ama-chromatogram e-HPLC kanye ne-COA anikezwa umkhiqizo oqediwe.