Uhlolojikelele nokusetshenziswa kwe-cerulein

Uhlolojikelele

I-Caerulein, eyaziwa nangokuthi i-cerulein, iyisikhumba sesele lase-Australia i-HYlacaerulea ehlanganisa ama-amino acid ayi-10.Kuyi-molecule ye-decapeptide ehlinzekwa yi-trifluoroacetate esebenza njenge-analogue ye-cholecystokinin kumaseli e-pancreatic vesicular futhi ingaholela ekukhiqizweni kwenani elikhulu lama-enzyme okugaya kanye nejusi ye-pancreatic, okuholela ku-acute edematous pancreatitis.I-Cerutin ingasetshenziselwa ukutadisha amaprotheni e-Nf-κb up-regulation afana ne-intercellular adhesion molecule-1 (ICAM-1) izici ezihlobene nokuvuvukala ezifana ne-NADPH oxidase kanye no-Janus kinase mediated signal transduction.Isetshenziswe ngempumelelo ukusungula amamodeli e-acute pancreatitis kumagundane, amagundane, izinja, nama-hamster aseSyria (AP).Uketshezi olufakwa emthanjeni lusetshenziswa kangcono ngomjovo we-intravenous, dermal, noma we-intraperitoneal.Isetshenziswa kakhulu ekuhlolweni komtholampilo kwe-acute edematous pancreatitis, futhi ukuhlolwa kwe-in vitro kusetshenziswa kumamodeli amaseli.Ngaphezu kwalokho, isetshenziselwa ukuhlola ukusebenza kwe-gallbladder.

Uhlolojikelele nokusetshenziswa kwe-cerulein

Imininingwane enemininingwane

Ukubukeka: impushana emhlophe

Inombolo ye-CAS: 17650-98-5

Inombolo ye-Gutuo: GT-F055

Ukulandelana: pGlu-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2

Ifomula yemolekyuli: C58H73N13O21S2

Isisindo se-molecule: 1352.4

Ukunyibilika: Incibilika ku-50mM ammonium hydroxide ekuhlanganiseni okungu-1.0mg/ml

Isicelo

1. Isetshenziswa kakhulu ekufanekiseni i-acute edematous pancreatitis ezivivinyweni zomtholampilo.

2. Isicelo samamodeli weselula ku-vitro.

3. Isetshenziselwa ukuhlola ukusebenza kwenyongo.

Ukwakha imodeli yocwaningo lwe-acute pancreatitis yocwaningo lwe-cerulein (AP) cell biology, izici ze-pathophysiological kanye nokubonakaliswa kwezifo eziphilayo ku-pancreatitis ebukhali.Ngaphezu kokuphenya izinguquko zamaphaphu zesifo se-AP, kungase futhi kubonise ngokuphumelelayo ukusebenzisana kwe-visceral endocrine njengezinga le-metabolin ne-CCK.Ingase futhi isetshenziselwe ukuhlola ukuvuselelwa nokubuyiselwa kwezicubu ezilimele ngemva kokuqedwa kwezinto eziyingozi.

5. Ukusetshenziswa kwe-Caerulein cerulein (cerulein) ne-LPS ukuze kusungulwe amamodeli e-pancreatitis kungaveza imiphumela ye-synergistic, eyokuqala ingashukumisa ama-enzyme e-pancreatic ukuze abhubhise ama-pancreas, futhi isebenze ngokuqhubekayo amangqamuzana avuthayo ukuze akhulule izici ezivuthayo.Kamuva, i-LPS iphazamisa ukusabela okuvamile kwabalamuli abavuvukalayo, ngaleyo ndlela kuthuthukiswe i-pancreatitis yasendaweni njengento ehleliwe yokuvuvukala okunamandla.

6. I-Cerulein ingasetshenziselwa ukuvimbela ubuhlungu be-gallbladder, i-renal colic, nobuhlungu be-claudication obuphakathi.Imvamisa ithathwa njengomphikisi ongapheli kephalin.