I-phosphorylation ithinta zonke izici zokuphila kwamaselula, futhi amaprotheni kinases athinta zonke izici zemisebenzi yokuxhumana ye-intracellular ngokulawula izindlela zokubonisa izimpawu kanye nezinqubo zamaselula.Nokho, i-phosphorylation engafanele futhi iyimbangela yezifo eziningi;ikakhulukazi, amaprotheni kinases aguquliwe kanye nama-phosphatase angabangela izifo eziningi, futhi ubuthi abaningi bemvelo kanye nama-pathogens nawo abe nomthelela ngokushintsha isimo se-phosphorylation samaprotheni e-intracellular.
I-Phosphorylation of serine (Ser), i-threonine (Thr), ne-tyrosine (Tyr) iyinqubo yokuguqulwa kwamaprotheni ebuyiseleka emuva.Bahileleke ekulawuleni imisebenzi eminingi yamaselula, njengokuboniswa kwe-receptor, ukuhlangana kwamaprotheni kanye nokuhlukaniswa, ukwenza kusebenze noma ukuvimbela ukusebenza kwamaprotheni, ngisho nokusinda kweseli.Ama-Phosphates akhokhiswa kabi (izinkokhelo ezimbili ezingezinhle ngeqembu le-phosphate ngalinye).Ngakho-ke, ukungezwa kwabo kuguqula izakhiwo zeprotheni, okuvame ukushintshwa okuhambisanayo, okuholela ekushintsheni kwesakhiwo seprotheni.Lapho iqembu le-phosphate lisuswa, ukuhlangana kwephrotheni kuzobuyela esimweni saso sokuqala.Uma amaphrotheni amabili e-conformational ekhombisa imisebenzi ehlukene, i-phosphorylation ingasebenza njengokushintshwa kwamangqamuzana ukuze iphrotheni ilawule umsebenzi wayo.
Amahomoni amaningi alawula umsebenzi wama-enzyme athile ngokwandisa isimo se-phosphorylation sezinsalela ze-serine (Ser) noma i-threonine (Thr), futhi i-tyrosine (Tyr) phosphorylation ingabangelwa izici zokukhula (njenge-insulin).Amaqembu e-phosphate alawa ma-amino acid angasuswa ngokushesha.Ngakho-ke, i-Ser, i-Thr, ne-Tyr isebenza njengokushintshwa kwamangqamuzana ekulawuleni imisebenzi yamaselula njengokwanda kwesimila.
Ama-peptide okwenziwa adlala indima ewusizo kakhulu ocwaningweni lwe-protein kinase substrates kanye nokusebenzisana.Nokho, kunezici ezithile ezithiya noma ezikhawulela ukuzivumelanisa nezimo kobuchwepheshe be-phosphopeptide synthesis, njengokungakwazi ukuzuza ukuzenzekelayo okugcwele kokuhlanganisa kwesigaba esiqinile kanye nokuntuleka kokuxhumana okulula nezinkundla zokuhlaziya ezijwayelekile.
Iplatifomu esekelwe ku-peptide synthesis kanye nobuchwepheshe bokuguqula i-phosphorylation iyanqoba le mikhawulo ngenkathi ithuthukisa ukusebenza kahle kokuhlanganiswa nokuqina, futhi inkundla ifaneleka kahle ukutadisha ama-protein kinase substrates, ama-antigen, ama-molecule abophayo, nama-inhibitors.
Isikhathi sokuthumela: May-31-2023